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Comparison of (A) retention times t R (HPLC, <t>C18),</t> (B) octanol/PBS partition coefficient log D 7.4 ± SD ( n = 3) (C) relative HSA binding ± SD ( n = 3) and (D) rhPSMA inhibition constants (IC 50 , n = 3) of the Cy5 dye conjugates 8 , 9 , 10 and the ZW800 conjugate 13 . Significant differences are indicated by asterisks (*** p < 0.001, ** p < 0.05).
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Comparison of (A) retention times t R (HPLC, <t>C18),</t> (B) octanol/PBS partition coefficient log D 7.4 ± SD ( n = 3) (C) relative HSA binding ± SD ( n = 3) and (D) rhPSMA inhibition constants (IC 50 , n = 3) of the Cy5 dye conjugates 8 , 9 , 10 and the ZW800 conjugate 13 . Significant differences are indicated by asterisks (*** p < 0.001, ** p < 0.05).
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Comparison of (A) retention times t R (HPLC, C18), (B) octanol/PBS partition coefficient log D 7.4 ± SD ( n = 3) (C) relative HSA binding ± SD ( n = 3) and (D) rhPSMA inhibition constants (IC 50 , n = 3) of the Cy5 dye conjugates 8 , 9 , 10 and the ZW800 conjugate 13 . Significant differences are indicated by asterisks (*** p < 0.001, ** p < 0.05).

Journal: Journal of Medicinal Chemistry

Article Title: Zwitterionic Modification of PSMA Ligands Reduces Off-Target Binding and Tissue Retention

doi: 10.1021/acs.jmedchem.5c03849

Figure Lengend Snippet: Comparison of (A) retention times t R (HPLC, C18), (B) octanol/PBS partition coefficient log D 7.4 ± SD ( n = 3) (C) relative HSA binding ± SD ( n = 3) and (D) rhPSMA inhibition constants (IC 50 , n = 3) of the Cy5 dye conjugates 8 , 9 , 10 and the ZW800 conjugate 13 . Significant differences are indicated by asterisks (*** p < 0.001, ** p < 0.05).

Article Snippet: Analytical HPLC-MS was performed on an Agilent HPLC system 1260 Infinity II with a Macherey-Nagel NUCLEODUR C18 Gravity-SB column (3 μm, 100 × 2 mm) coupled to a Bruker amaZon SL ion trap mass spectrometer with an ESI source.

Techniques: Comparison, Binding Assay, Inhibition